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KMID : 0613820040140040670
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Journal of Life Science
2004 Volume.14 No. 4 p.670 ~ p.675
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PGE2 Mediated INF-γ Gene Methylation Through cAMP Signaling Pathway in Human Jurkat T Cells
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Jeon Byung-Hoon
Kim Myung-Wan
Yun Young-Gab
Park Hyun
Chung Hun-Taeg
Han Dong-Min
Kim Won-Sin
Ju Sung-Min
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Abstract
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We have examined the effects of S-nitroso-N-acetylpenicillamine (SNAP), prostaglandin E2 (PGE2) and dibutric cyclic AMP (dbcAMP) on the methylation of interferon-γ (IFN-γ) gene in human Jurkat T cells. The CpG dinucleotide which is critical for promoter function of IFN-γ gene was methylated by treatment with SNAP, PGE2, and dbcAMP, respectively. The DNA methylation induced by PGE2 was suppressed by the addition of 2’,5’-dideoxyadenosine (DDA), an inhibitor of adenylyl cyclase, but the suppression was not observed in SNAP treated cells. The NO production was not enhanced in PGE2 or dbcAMP treated cells. The methylation induced by PGE2 and dbcAMP was not suppressed by the addition of NG-methyl-L-arginine (L-NMMA), NO synthase inhibitor. In conclusion, the inhibition of INF-γ gene expression by PGE2 was associated with the methylation of INF-γ gene by elevation of intracellular cAMP in human Jurkat T cells. However, the methylation induced by PGE2 might not be mediated through the NO production
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KEYWORD
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INF-γ, CpG dinucleotide, Methylation, Nitric oxide, PGE2
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